B95 8 Cell Line
In some instances the cell line b95 8 has been described as derived from marmoset however this is not correct.
B95 8 cell line. Marmoset used to be described in the animal name section was a mistake and corrected to the name cotton top tamarin with the scientific name. 94 100 based on 2 pubmed citations. The b95 8 cell line must be handled in the bsl 2 labotory since ebv can infect primates. Fish and wildlife service.
The ebv producing marmoset b cell line b95 8 commonly used as a source of ebv for stimulation and transformation of human b cells was shown to proliferate in response to supernatants containing human b cell growth factors bcgf derived from pha activated t cells or the kg la cell line and to a commercial low molecular weight bcgf bcgflow but not to recombinant human il 4. B95 8 cell line flask presentation1 flask 75 cm2 ftb95 information request. B95 8 monkey marmoset lymphocyte b peripheral species. B95 8 was derived from a cotton top tamarin saguinas oedipus.
Releases high titres of transforming ebv. This has been confirmed by dna profiling. Ebv providing a source for transformation of b cells and establishment of continuous cell lines. Zero bias scores article reviews protocol conditions and more.
The cells should be handled under laboratory containment level 2. The b95 8 cell line s name originates from the b95 strain of epstein barr virus ebv. Marmoset used to be described in the animal name section was a mistake and corrected to the name cotton top tamarin with the scientific name. The b95 8 cell line must be handled in the p2 labotory since ebv can infect primates.
This cell line has been assigned by the zkbs german central commission for biological safety to risk group 2 ief analysis with ldh and np confirmed the cells as non. This cell line is used as a source of ebv. The viral strain was isolatedfrom a case of transfusion induced mononucleosis and subsequently used in vitro to immortalise monkey blood leukocytes miller et al pnas 1972 to generate the cell line. The cells should be handled under laboratory containment level 2.
Through the use of dna profiling it has now been shown. However the cell line is derived from a primate species that is listed as endangered by the u s. If you need more information about the product please fill in the form. In some instances the cell line b95 8 has been described as.
B95 8 b95 8 b958 cotton top tamarin monkey peripheral blood lymphocyte derived from a cotton top tamarin saguinus oedipus. Thus it provides a source of ebv to establish continuous lymphocytic lines from human donors.